PPI network (data source: BioGrid)

Symbol A GeneA coding DNM count Symbol B GeneB coding DNM count Interaction detection method Publication Evidence
TK1 0 CDKN1A 1 affinity chromatography technology, pull down Huang DY (2001), Huang DY (2001) 2
SIAH1 2 GAPDH 0 affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology Lee SY (2012), Lee SB (2012), Tristan CA (2015), Suarez S (2015) 4
SNCA 0 PRDX1 1 unspecified method Woods WS (2007) 1
TRIM25 5 PKM 1 affinity chromatography technology, affinity chromatography technology Choudhury NR (2017), Choudhury NR (2017) 2
EGFR 5 GAPDH 0 affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, two hybrid, protein complementation assay Foerster S (2013), Wu SL (2006), Wu SL (2006), Kotlyar M (2015), Petschnigg J (2014) 5
FN1 17 EEF1A1 0 affinity chromatography technology Humphries JD (2009) 1
CDKN1A 1 EEF1A1 0 two hybrid Vinayagam A (2011) 1
CCNB1 3 EP300 36 pull down, affinity chromatography technology Felzien LK (1998), Felzien LK (1998) 2
PRDX1 1 GAPDH 0 two hybrid, biochemical Stelzl U (2005), Wan C (2015) 2
GAPDH 0 NPM1 0 affinity chromatography technology, affinity chromatography technology, affinity chromatography technology Lee SB (2012), Lee SB (2012), Chen R (2013) 3
FN1 17 ENO1 3 affinity chromatography technology Humphries JD (2009) 1
EEF1A1 0 HSP90AA1 7 affinity chromatography technology Aranda-Orgilles B (2008) 1
TRIM25 5 HSP90AA1 7 affinity chromatography technology Choudhury NR (2017) 1
EGFR 5 NPM1 0 affinity chromatography technology Liccardi G (2014) 1
MYOC 1 GAPDH 0 two hybrid, far western blotting Fautsch MP (2006), Fautsch MP (2006) 2
PCNA 0 ENO1 3 affinity chromatography technology, pull down Naryzhny SN (2010), Naryzhny SN (2010) 2
GAPDH 0 EP300 36 affinity chromatography technology, affinity chromatography technology Sen N (2008), Sen N (2008) 2
HSP90AB1 2 GAPDH 0 affinity chromatography technology, biochemical Falsone SF (2005), Wan C (2015) 2
TRIM25 5 APP 1 affinity chromatography technology Choudhury NR (2017) 1
TK1 0 PKM 1 two hybrid Vinayagam A (2011) 1
HSP90AA1 7 PKM 1 affinity chromatography technology Falsone SF (2005) 1
HSP90AA1 7 APP 1 affinity chromatography technology Kumar P (2007) 1
FN1 17 TRAP1 0 affinity chromatography technology Humphries JD (2009) 1
GAPDH 0 AR 3 two hybrid, pull down Koshy B (1996), Koshy B (1996) 2
CCNB1 3 CDKN1A 1 affinity chromatography technology, pull down, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, imaging technique Amador V (2007), Garcia-Wilson E (2005), Ando T (2001), Poon RY (1996), Fogeron ML (2013), Chen TC (2014) 6
CCNB1 3 PCNA 0 affinity chromatography technology, affinity chromatography technology Zhang H (1993), Huttlin EL (2017) 2
TRIM25 5 EP300 36 affinity chromatography technology, affinity chromatography technology Choudhury NR (2017), Choudhury NR (2017) 2
NPM1 0 ENO1 3 affinity chromatography technology Chen R (2013) 1
PRKCI 1 HSP90AA1 7 affinity chromatography technology, affinity chromatography technology Brajenovic M (2004), Taipale M (2012) 2
ASPSCR1 3 APP 1 pull down Olah J (2011) 1
EEF1A1 0 PKM 1 biochemical Wan C (2015) 1
RPA2 0 EGFR 5 affinity chromatography technology Marechal A (2014) 1
FN1 17 GAPDH 0 affinity chromatography technology, affinity chromatography technology Humphries JD (2009), Moussavi-Harami SF (2013) 2
EGFR 5 SNCA 0 two hybrid, protein complementation assay, affinity chromatography technology Kotlyar M (2015), Petschnigg J (2014), Petschnigg J (2014) 3
TRAP1 0 CCNB1 3 affinity chromatography technology Sisinni L (2017) 1
PRKN 1 HSP90AA1 7 affinity chromatography technology, affinity chromatography technology, affinity chromatography technology Imai Y (2003), Taipale M (2012), Zanon A (2013) 3
GRIA2 4 GAPDH 0 affinity chromatography technology, affinity chromatography technology, pull down Wang M (2012), Wang M (2012), Wang M (2012) 3
EGFR 5 HSP90AA1 7 affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, two hybrid, protein complementation assay Citri A (2002), Yang S (2006), Shimamura T (2005), Lavictoire SJ (2003), Lavictoire SJ (2003), Foerster S (2013), Kotlyar M (2015), Petschnigg J (2014) 8
CDKN1A 1 SET 8 pull down, pull down Canela N (2003), Carujo S (2006) 2
SIRT1 3 HSP90AA1 7 affinity chromatography technology, affinity chromatography technology Lin Z (2012), Hein MY (2015) 2
PCNA 0 EGFR 5 affinity chromatography technology, affinity chromatography technology, enzymatic study Wang SC (2006), Wang SC (2006), Wang SC (2006) 3
SIAH1 2 NPM1 0 affinity chromatography technology, affinity chromatography technology Lee SB (2012), Lee SB (2012) 2
TRIM25 5 GAPDH 0 affinity chromatography technology, affinity chromatography technology Choudhury NR (2017), Choudhury NR (2017) 2
SIRT1 3 CCNB1 3 affinity chromatography technology Sasaki T (2008) 1
TRAP1 0 GAPDH 0 biochemical, biochemical Matassa DS (2013), Wan C (2015) 2
TRIM25 5 TPI1 1 affinity chromatography technology Choudhury NR (2017) 1
EEF1A1 0 GAPDH 0 biochemical, biochemical, biochemical Havugimana PC (2012), Kristensen AR (2012), Wan C (2015) 3
SNCA 0 EEF1A1 0 two hybrid Vinayagam A (2011) 1
HSP90AA1 7 SNCA 0 pull down Falsone SF (2009) 1
FN1 17 PCNA 0 affinity chromatography technology Humphries JD (2009) 1
FN1 17 HSP90AA1 7 affinity chromatography technology Humphries JD (2009) 1
FN1 17 NPM1 0 affinity chromatography technology Humphries JD (2009) 1
SET 8 CCNB1 3 affinity chromatography technology Carujo S (2006) 1
GAPDH 0 PKM 1 biochemical, biochemical Havugimana PC (2012), Wan C (2015) 2
RPA2 0 GAPDH 0 two hybrid, affinity chromatography technology, affinity chromatography technology Stelzl U (2005), Stelzl U (2005), Marechal A (2014) 3
MAPK7 1 PKM 1 affinity chromatography technology Erazo T (2013) 1
NPM1 0 HSP90AB1 2 affinity chromatography technology Chen R (2013) 1
HSP90AB1 2 PGK1 1 affinity chromatography technology Falsone SF (2005) 1
FN1 17 TK1 0 affinity chromatography technology Humphries JD (2009) 1
MYOC 1 ENO1 3 far western blotting Fautsch MP (2006) 1
POU2F1 5 AR 3 pull down Prefontaine GG (1999) 1
HSP90AA1 7 MAPK7 1 affinity chromatography technology Taipale M (2012) 1
NPM1 0 HSP90AA1 7 affinity chromatography technology Chen R (2013) 1
PGK1 1 TPI1 1 biochemical, biochemical Havugimana PC (2012), Wan C (2015) 2
FN1 17 PRDX1 1 affinity chromatography technology Humphries JD (2009) 1
TPI1 1 APP 1 pull down Olah J (2011) 1
PKM 1 PRKN 1 affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, pull down, pull down, enzymatic study Liu K (2016), Liu K (2016), Liu K (2016), Liu K (2016), Liu K (2016), Liu K (2016) 6
GAPDH 0 GAPDHS 1 affinity chromatography technology, affinity chromatography technology Huttlin EL (2015), Huttlin EL (2017) 2
FN1 17 RPA2 0 affinity chromatography technology Humphries JD (2009) 1
CDKN1A 1 EP300 36 pull down, affinity chromatography technology Cazzalini O (2008), Cazzalini O (2008) 2
GAPDH 0 ATXN1 2 two hybrid, pull down Koshy B (1996), Koshy B (1996) 2
CCNB1 3 PRDX1 1 affinity chromatography technology Hein MY (2015) 1
TRIM25 5 PGAM1 0 affinity chromatography technology Choudhury NR (2017) 1
EGFR 5 PRDX1 1 protein complementation assay, affinity chromatography technology, affinity chromatography technology Deribe YL (2009), Tong J (2008), Foerster S (2013) 3
PRKN 1 SET 8 affinity chromatography technology Zanon A (2013) 1
TRIM25 5 PCNA 0 affinity chromatography technology, affinity chromatography technology Park JM (2014), Park JM (2014) 2
APP 1 SNCA 0 pull down, pull down, affinity chromatography technology, affinity chromatography technology, pull down, pull down, imaging technique Jensen PH (1997), Yoshimoto M (1995), Tsigelny IF (2008), Tsigelny IF (2008), Tsigelny IF (2008), Olah J (2011), Chen TC (2014) 7
EP300 36 PCNA 0 affinity chromatography technology, affinity chromatography technology, pull down, pull down, affinity chromatography technology, affinity chromatography technology, pull down, affinity chromatography technology, affinity chromatography technology, enzymatic study Hasan S (2001), Hasan S (2001), Hasan S (2001), Hong R (2003), Hong R (2003), Cazzalini O (2008), Cazzalini O (2008), Naryzhny SN (2004), Cazzalini O (2014), Cazzalini O (2014) 10
PRKN 1 APP 1 affinity chromatography technology, imaging technique Rosen KM (2010), Lonskaya I (2012) 2
EGFR 5 FN1 17 protein complementation assay Deribe YL (2009) 1
PRKCI 1 APP 1 pull down Olah J (2011) 1
EGFR 5 TPI1 1 protein complementation assay Deribe YL (2009) 1
SIAH1 2 SNCA 0 pull down, affinity chromatography technology, enzymatic study, affinity chromatography technology, pull down, affinity chromatography technology, enzymatic study, affinity chromatography technology, affinity chromatography technology Lee JT (2008), Lee JT (2008), Rott R (2008), Rott R (2008), Szargel R (2009), Szargel R (2009), Szargel R (2009), Szargel R (2009), Szargel R (2016) 9
TK1 0 GAPDH 0 two hybrid, two hybrid Stelzl U (2005), Vinayagam A (2011) 2
NPM1 0 EEF1A1 0 affinity chromatography technology Chen R (2013) 1
MAPK7 1 APP 1 pull down, pull down Olah J (2011), Virok DP (2011) 2
EGFR 5 PLD2 1 affinity chromatography technology Slaaby R (1998) 1
PKM 1 TRAP1 0 biochemical Wan C (2015) 1
PCNA 0 TPI1 1 pull down Naryzhny SN (2010) 1
SLC2A4 1 HSP90AA1 7 affinity chromatography technology Foster LJ (2006) 1
GAPDH 0 PGK1 1 biochemical, biochemical, affinity chromatography technology Havugimana PC (2012), Wan C (2015), Fokina KV (1997) 3
POU2F1 5 NPAT 4 affinity chromatography technology, affinity chromatography technology Zheng L (2003), Zheng L (2003) 2
GAPDH 0 CCNB1 3 pull down, affinity chromatography technology Carujo S (2006), Carujo S (2006) 2
GAPDH 0 PGAM1 0 biochemical, biochemical Havugimana PC (2012), Wan C (2015) 2
PRKN 1 EEF1A1 0 affinity chromatography technology Zanon A (2013) 1
TRIM25 5 PRDX1 1 affinity chromatography technology, affinity chromatography technology Choudhury NR (2017), Choudhury NR (2017) 2
GAPDH 0 ASPSCR1 3 affinity chromatography technology, affinity chromatography technology Huttlin EL (2015), Huttlin EL (2017) 2
PCNA 0 EEF1A1 0 affinity chromatography technology Naryzhny SN (2010) 1
NPM1 0 APP 1 pull down Olah J (2011) 1
TRIM25 5 POU2F1 5 affinity chromatography technology Choudhury NR (2017) 1
MYOC 1 PKM 1 two hybrid Fautsch MP (2006) 1
PCNA 0 PGAM1 0 affinity chromatography technology, pull down Naryzhny SN (2010), Naryzhny SN (2010) 2
AR 3 EP300 36 affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, enzymatic study, affinity chromatography technology, affinity chromatography technology Dai Y (2008), Minges JT (2013), Zhong J (2014), Fu M (2000), McClurg UL (2016), Tan G (2017) 6
HSP90AA1 7 GAPDH 0 affinity chromatography technology, biochemical Falsone SF (2005), Wan C (2015) 2
POU2F1 5 GAPDH 0 pull down, affinity chromatography technology, affinity chromatography technology Zheng L (2003), Zheng L (2003), Zheng L (2003) 3
GAPDH 0 GAPDH 0 x-ray crystallography, two hybrid, pull down Jenkins JL (2006), Wang J (2011), Torres-Bugeau CM (2012) 3
EGFR 5 APP 1 two hybrid, protein complementation assay Kotlyar M (2015), Petschnigg J (2014) 2
SNCA 0 GAPDH 0 two hybrid, pull down Vinayagam A (2011), Tsuchiya K (2005) 2
PRKN 1 SNCA 0 affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, genetic interference, genetic interference, affinity chromatography technology Choi P (2001), Choi P (2001), Kawahara K (2008), Hampe C (2006), Nemes Z (2009), Oluwatosin-Chigbu Y (2003), Yang Y (2003), Norris KL (2015) 8
MYOC 1 FN1 17 pull down Filla MS (2002) 1
CDKN1A 1 PCNA 0 two hybrid, pull down, pull down, pull down, pull down, x-ray crystallography, pull down, two hybrid, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, pull down, pull down, affinity chromatography technology, biochemical, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, unspecified method, pull down, affinity chromatography technology, biochemical, pull down, affinity chromatography technology, affinity chromatography technology, two hybrid, unspecified method, affinity chromatography technology, biochemical, two hybrid, affinity chromatography technology, two hybrid, pull down, affinity chromatography technology, affinity chromatography technology, pull down, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, pull down, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology Rual JF (2005), Frouin I (2003), Watanabe H (1998), Fotedar R (1996), Jonsson ZO (1998), Gulbis JM (1996), Touitou R (2001), Yu P (2001), Yu P (2001), Chen X (2007), Chen X (2007), Wang SC (2006), Carujo S (2006), Garcia-Wilson E (2005), Howlett NG (2009), Jin Y (2008), Lee JY (2009), Ando T (2001), Ando T (2001), Takasaki Y (2001), Logette E (2011), Mistafa O (2010), Mistafa O (2010), Bruning JB (2004), Punchihewa C (2012), Mattock H (2001), Waga S (1994), Waga S (1994), Poon RY (1996), Zhang Y (1999), Warbrick E (1995), Warbrick E (1995), Poon RY (1998), Poon RY (1998), Wang J (2011), Fogeron ML (2013), Rolland T (2014), Rizzardi LF (2014), Trombetta A (2013), Trombetta A (2013), Kim DH (2010), Huttlin EL (2015), Hein MY (2015), Hein MY (2015), Coleman KE (2015), Huttlin EL (2017), Roessig L (2001), Roessig L (2001) 48
PRKN 1 HSP90AB1 2 affinity chromatography technology Zanon A (2013) 1
GAPDH 0 SET 8 pull down, affinity chromatography technology Carujo S (2006), Carujo S (2006) 2
RPA2 0 PRDX1 1 affinity chromatography technology Marechal A (2014) 1
EP300 36 PKM 1 affinity chromatography technology, imaging technique Luo W (2011), Luo W (2011) 2
GAPDH 0 APP 1 pull down, pull down Olah J (2011), Virok DP (2011) 2
POU2F1 5 APP 1 pull down Olah J (2011) 1
PRKN 1 EGFR 5 affinity chromatography technology, affinity chromatography technology Lin DC (2015), Lin DC (2015) 2
TK1 0 EEF1A1 0 two hybrid Vinayagam A (2011) 1
EP300 36 SIRT1 3 affinity chromatography technology, affinity chromatography technology Bouras T (2005), Bourguignon LY (2009) 2
PRKN 1 CCNB1 3 affinity chromatography technology, affinity chromatography technology, enzymatic study Lee SB (2015), Lee SB (2015), Lee SB (2015) 3
HSP90AA1 7 PGK1 1 affinity chromatography technology Falsone SF (2005) 1
PCNA 0 RPA2 0 affinity chromatography technology Takasaki Y (2001) 1
EGFR 5 EEF1A1 0 affinity chromatography technology Foerster S (2013) 1
CDKN1A 1 GAPDH 0 affinity chromatography technology, pull down Carujo S (2006), Carujo S (2006) 2
CDKN1A 1 APP 1 pull down Olah J (2011) 1
HSP90AB1 2 PKM 1 affinity chromatography technology Falsone SF (2005) 1
ENO1 3 PGAM1 0 biochemical Wan C (2015) 1
PRKN 1 PCNA 0 affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology Kao SY (2009), Kao SY (2009), Zhu X (2017), Zhu X (2017), Zhu X (2017) 5
HSP90AA1 7 AR 3 affinity chromatography technology, pull down, affinity chromatography technology, affinity chromatography technology, pull down, affinity chromatography technology, two hybrid, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology Veldscholte J (1992), Nemoto T (1992), Zoubeidi A (2007), DaSilva J (2009), Chymkowitch P (2011), Gibbs A (2009), Wolf SS (2007), Luo J (2015), Kwegyir-Afful AK (2015), Ni L (2010), McClurg UL (2016) 11
HSP90AB1 2 HSP90AA1 7 affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, biochemical, affinity chromatography technology, affinity chromatography technology, biochemical, affinity chromatography technology Cheng MB (2010), Gano JJ (2010), Skarra DV (2011), Kristensen AR (2012), Taipale M (2014), Taipale M (2014), Wan C (2015), Hein MY (2015) 8
PCNA 0 PGK1 1 affinity chromatography technology, pull down Naryzhny SN (2010), Naryzhny SN (2010) 2
CDKN1A 1 NPM1 0 affinity chromatography technology, affinity chromatography technology, pull down, imaging technique, affinity chromatography technology Xiao J (2009), Xiao J (2009), Xiao J (2009), Xiao J (2009), Li J (2005) 5
SET 8 APP 1 unspecified method, affinity chromatography technology Madeira A (2005), Briand S (2011) 2
PKM 1 APP 1 pull down Olah J (2011) 1
ENO1 3 TPI1 1 biochemical, biochemical Havugimana PC (2012), Wan C (2015) 2
EP300 36 EEF1A1 0 pull down Karmakar S (2010) 1
TRIM25 5 PRKCI 1 affinity chromatography technology Choudhury NR (2017) 1
PRKN 1 PRDX1 1 affinity chromatography technology Zanon A (2013) 1
SIRT1 3 GAPDH 0 affinity chromatography technology, pull down Kornberg MD (2010), Kornberg MD (2010) 2
PCNA 0 GAPDH 0 affinity chromatography technology, pull down Naryzhny SN (2010), Naryzhny SN (2010) 2
FN1 17 PKM 1 affinity chromatography technology Humphries JD (2009) 1
RPA2 0 NPM1 0 affinity chromatography technology Marechal A (2014) 1
TRAP1 0 EEF1A1 0 affinity chromatography technology, affinity chromatography technology Matassa DS (2013), Matassa DS (2013) 2
SLC2A4 1 GAPDH 0 affinity chromatography technology, affinity chromatography technology Foster LJ (2006), Foster LJ (2006) 2
RPA2 0 EEF1A1 0 two hybrid, affinity chromatography technology Stelzl U (2005), Marechal A (2014) 2
PRKN 1 NPM1 0 affinity chromatography technology Zanon A (2013) 1
EGFR 5 HSP90AB1 2 affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, two hybrid, protein complementation assay, affinity chromatography technology Lavictoire SJ (2003), Lavictoire SJ (2003), Foerster S (2013), Kotlyar M (2015), Petschnigg J (2014), Petschnigg J (2014) 6
PRKN 1 GAPDH 0 biochemical, affinity chromatography technology Schlehe JS (2008), Zanon A (2013) 2
MAPK7 1 HSP90AB1 2 affinity chromatography technology, affinity chromatography technology, affinity chromatography technology Erazo T (2013), Erazo T (2013), Erazo T (2013) 3
GAPDH 0 TPI1 1 biochemical, biochemical Havugimana PC (2012), Wan C (2015) 2
SIRT1 3 AR 3 affinity chromatography technology, affinity chromatography technology, pull down, enzymatic study Fu M (2006), Fu M (2006), Fu M (2006), Fu M (2006) 4
NPM1 0 PKM 1 affinity chromatography technology Chen R (2013) 1
EP300 36 NPM1 0 enzymatic study Swaminathan V (2005) 1
PLD2 1 GAPDH 0 affinity chromatography technology, pull down Kim JH (2003), Kim JH (2003) 2
TRAP1 0 APP 1 pull down Olah J (2011) 1
RPA2 0 APP 1 pull down Olah J (2011) 1
MAPK7 1 GAPDH 0 affinity chromatography technology, affinity chromatography technology Erazo T (2013), Varjosalo M (2013) 2
TK1 0 APP 1 pull down Olah J (2011) 1
EGFR 5 AR 3 affinity chromatography technology, affinity chromatography technology, imaging technique, imaging technique Bonaccorsi L (2004), Bonaccorsi L (2004), Bonaccorsi L (2004), Bonaccorsi L (2004) 4
TRIM25 5 ENO1 3 affinity chromatography technology, affinity chromatography technology Choudhury NR (2017), Choudhury NR (2017) 2
PKM 1 PGK1 1 biochemical Wan C (2015) 1
FN1 17 SET 8 affinity chromatography technology Humphries JD (2009) 1
CDKN1A 1 PKM 1 two hybrid Vinayagam A (2011) 1
FN1 17 HSP90AB1 2 affinity chromatography technology Humphries JD (2009) 1
TRIM25 5 EEF1A1 0 biochemical Wan C (2015) 1
GAPDH 0 NPAT 4 affinity chromatography technology, affinity chromatography technology Zheng L (2003), Zheng L (2003) 2
TRIM25 5 PGK1 1 affinity chromatography technology Choudhury NR (2017) 1
MYOC 1 EEF1A1 0 two hybrid Fautsch MP (2006) 1
HSP90AA1 7 CDKN1A 1 affinity chromatography technology Jascur T (2005) 1
PRKCI 1 GAPDH 0 enzymatic study, affinity chromatography technology Tisdale EJ (2002), Tisdale EJ (2002) 2
PGK1 1 EEF1A1 0 biochemical Wan C (2015) 1
ENO1 3 PGK1 1 biochemical, biochemical Havugimana PC (2012), Wan C (2015) 2
GAPDHS 1 APP 1 pull down Olah J (2011) 1
ENO1 3 GAPDH 0 biochemical, biochemical Havugimana PC (2012), Wan C (2015) 2
FN1 17 PGK1 1 affinity chromatography technology Humphries JD (2009) 1
PGAM1 0 TPI1 1 biochemical Wan C (2015) 1
NPM1 0 PRKCI 1 affinity chromatography technology Chen R (2013) 1
NPM1 0 SIRT1 3 affinity chromatography technology Park G (2010) 1